Journal: Acta Neuropathologica Communications

Article Title: Higher levels of myelin phospholipids in brains of neuronal α-Synuclein transgenic mice precede myelin loss

doi: 10.1186/s40478-017-0439-3

Figure Lengend Snippet: Higher levels of phospholipids in purified myelin from neuronal α-Syn-expressing mouse brains. a 31 P NMR spectra of a sample consisting of a chloroform/methanol extract of purified myelin obtained from a Thy-1 human wt α-Syn tg mouse brain. The 31 P NMR spectra were obtained with a 500 MHz NMR spectrometer (Bruker, Germany) with a 5 mm broadband probe. The 31 P chemical shifts were assigned according to standard phosphatidic acid (PA) and phosphatidylserine (PS), spiked into the sample and referenced to phosphatidylcholine (PC) pick at 0 ppm. b The area under the curves was calculated for samples of A53T α-Syn and control mouse brains and the relative amount of specific phospholipids was calculated using the MNova data processing program (Mestrelab Research); mean ± SD of n = 5 mouse brains; *, < 0.05, one-way ANOVA. phosphatidylethanolamine plasmalogen (PE-plasm); phosphatidylethanolamine (PE); sphingomyelin (SPH); phosphatidylinositol (PI); phosphatidylcholin (PC)

Article Snippet: The 31 P chemical shifts were assigned according to standard phosphatidic acid (PA) and phosphatidylserine (PS), spiked into the sample and referenced to phosphatidylcholine (PC) pick at 0 ppm. b The area under the curves was calculated for samples of A53T α-Syn and control mouse brains and the relative amount of specific phospholipids was calculated using the MNova data processing program (Mestrelab Research); mean ± SD of n = 5 mouse brains; *, < 0.05, one-way ANOVA. phosphatidylethanolamine plasmalogen (PE-plasm); phosphatidylethanolamine (PE); sphingomyelin (SPH); phosphatidylinositol (PI); phosphatidylcholin (PC) In order to confirm the effect of α-Syn overexpression on the phospholipid content of myelin, we employed a second mouse model, Thy-1 human wt α-Syn transgenic mice.

Techniques: Purification, Expressing, Control

Journal: PLoS ONE

Article Title: Analysis of the Cerebrospinal Fluid Proteome in Alzheimer's Disease

doi: 10.1371/journal.pone.0150672

Figure Lengend Snippet: (A) Comparison of identification performance between five mass spectrometry data processing tools based on number of identified peptides, proteins, and proteins identified with more than one peptide. (B) The number of identified and mapped proteins across the different programs. (C) Overlap of protein identification between different programs.

Article Snippet: The following five mass spectrometry data processing programs were used to perform identification and quantification: Sieve v 2.1 (Thermo), DecyderMS v2.0 (GE healthcare), Maxquant [ ], PEAKS (Bioinformatics Solutions Inc.) and OpenMS [ ].

Techniques: Comparison, Mass Spectrometry

Journal: PLoS ONE

Article Title: Analysis of the Cerebrospinal Fluid Proteome in Alzheimer's Disease

doi: 10.1371/journal.pone.0150672

Figure Lengend Snippet: Comparison of the number of significantly altered proteins identified by different normalization methods and five mass spectrometry data processing tools.

Article Snippet: The following five mass spectrometry data processing programs were used to perform identification and quantification: Sieve v 2.1 (Thermo), DecyderMS v2.0 (GE healthcare), Maxquant [ ], PEAKS (Bioinformatics Solutions Inc.) and OpenMS [ ].

Techniques: Comparison, Mass Spectrometry

Journal: PLoS ONE

Article Title: Analysis of the Cerebrospinal Fluid Proteome in Alzheimer's Disease

doi: 10.1371/journal.pone.0150672

Figure Lengend Snippet: (A) Scatter plot of protein fold changes between mass spectrometry (reference normalization) and antibody-based analysis. The protein p-values and fold changes were calucluated using statistically significantly differentially altered peptides. (B) Scatter plot of protein fold changes between mass spectrometry (spiked-in normalization) and antibody-based profiling. After normalization with spiked-in method, the protein p-values and fold changes were computed using the same peptides as used in the panel A (C) Scatter plot of protein fold changes between mass spectrometry (spiked-in normalization) and a where the protein p-values and fold changes were computed using statistically significantly differentially altered peptides (proteins are shown based on Uniprot ID). MS: Mass spectrometry; ABA: antibody-based analysis.

Article Snippet: The following five mass spectrometry data processing programs were used to perform identification and quantification: Sieve v 2.1 (Thermo), DecyderMS v2.0 (GE healthcare), Maxquant [ ], PEAKS (Bioinformatics Solutions Inc.) and OpenMS [ ].

Techniques: Mass Spectrometry

Journal: PLoS ONE

Article Title: Analysis of the Cerebrospinal Fluid Proteome in Alzheimer's Disease

doi: 10.1371/journal.pone.0150672

Figure Lengend Snippet: Proteins selected from spiked-in normalized data that were verified in the antibody-based analysis as novel disease-associated markers for Alzheimer’s disease.

Article Snippet: The following five mass spectrometry data processing programs were used to perform identification and quantification: Sieve v 2.1 (Thermo), DecyderMS v2.0 (GE healthcare), Maxquant [ ], PEAKS (Bioinformatics Solutions Inc.) and OpenMS [ ].

Techniques: Mass Spectrometry